Services
These techniques will be adapted according to the specimen and the scientific question.
Standard preparation of samples
Chemical fixation
Resin embedding
Ultramicrotomy (Semi-thin and ultra-thin sections + positive staining)
Sample preparation for correlative microscopy (CLEM)
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Immunocytochemistry
Manual immunolabelling
Automated immunolabelling
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Staining on grid of singles objects (molecules, particles …)
Negative staining
Shadowing
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Cryomethods
High Pressure Freezing
Freeze substitution (manual or automated)
Progressive Lowering of Temperature (PLT)
Freezing in ethane (cryoplunge)
Cryo-sectioning of chemically fixed samples: Tokuyasu technique
Cryo-sectioning of vitreous samples: CEMOVIS technique
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Grids processing
Preparation of coated grids
(formvar, collodion, carbon)
Glow discharge
Carbon or metal evaporation
Unidirectional shadowing
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TEM observations
High Contrast mode
Cryo-microscopy
Electron tomography
Energy filtered imaging (EFTEM/EELS)
(Cryo) Electronic tomography
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